基于16S rRNA基因的鼠Mycoplasma haemomuris的分子鉴定和种系发育分析

doi:10.11843/j.issn.0366-6964.2015.08.025

Abstract

Abstract:

This experiment was conducted to study the species characteristic and phylogenetic relationship of hemoplasmas from the wild rats on molecular level.Thirty-two blood samples collected from wild Rattus edwardsi in Hunan and Guangxi province of China were investigated by PCR aplication based on the 16S rRNA gene sequence.The total infection rate of hemoplasmas was 65.63% (21/32).Genetic variations showed that the inter-specific differences between M.haemomuris isolates and other Mycoplasma species (3.0%-4.5%) were bigger than that (0.8%) between two synonymic species (Haemobartonella muris and M.haemomuris).Phylogenetic analyses indicated that M.haemomuris derived from wild Rattus edwardsi grouped into a solitary clade closely related to H.muris and M.haemomuris. The results suggested that M.haemomuris isolated in the present study should represent a new genotype，and temporarily named M. haemomuris Rattus edwardsi strain.These data may have important implications for researching epidemiology and population biology as well as for studying the taxonomy and status of the genus Mycoplasma.

CLC Number:

S852.62

BAI Ai-quan，LI Gao-qiang，GUO Jian-chao，LI Xin，PU Wen-jun，LI Guo-qing，CHEN Zhi-wei，ZHANG Hao-ji. Molecular Identification and Phylogenetic Analysis of Mycoplasma haemomuris Isolates from Wild Rattus edwardsi based on 16S Ribosomal RNA Gene[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(8): 1471-1476.

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Molecular Identification and Phylogenetic Analysis of Mycoplasma haemomuris Isolates from Wild Rattus edwardsi based on 16S Ribosomal RNA Gene

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